How is the process of translation initiation different between eukaryotes and prokaryotes? 1) Eukaryotes have a 5' cap on mRNA, while prokaryotes do not. 2) Eukaryotes have a ribosome binding site on mRNA, while prokaryotes do not. 3) Eukaryotes require initiation factors for translation initiation, while prokaryotes do not. 4) Eukaryotes have a larger ribosome subunit, while prokaryotes have a smaller ribosome subunit.